Wpi monitors have both resolution and accuracy of 01c in the 0 50c range and are traceable to nist standards whereas other competitive electronic thermometers have an accuracy that is usually to 05c or worse. Among studies involving core and surface temperature measurements in mice the surface temperature is on average 257 c 34 c or 23 c lower than core temperature depending on the site of.
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Mouse body temperature measurement. Hi karin you could use passive infrared transponder pit tags that include a temperature sensor injected under the skin to measure subcutaneous body temperature a couple of companies make these. We show that body surface temperatures are about 20 c lower than rectal probe measurements but the. Body temperature is a measure of your bodys ability to make and get rid of heat. Place the mouse in an anesthesia induction box. This method of body temperature measurement is successful in reliably replicating temperature change trends during passive system anaphylaxis experiments in mice. Temperatures at ranges between 2 and 150 mm.
In fact the temperature measured by this method is one of highest temperatures in the body of endotherms corresponding to deep core body temperature donhoffer 1980. 1 inserting a probe into the rectum or less typically into the sigmoid colon. When precise temperature measurements are required wpi can provide you with a very accurate monitor and thermocouple microprobes. Thermocouples thermistors and rtds. Twenty four hour brown adipose tissue bat thermometry and abdominal body temperature readings in a freely moving c57bl6 mouse maintained at 26 c. 2 measuring temperature in the abdominal.
Anesthetize by using 1 lmin flow of oxygen with 5 isoflurane. When you are too hot the blood vessels in your skin widen to carry the excess heat to your skins surface. Anesthetization is confirmed when the mouse ceases voluntary movement and has been immobile for over 30 s. You may start to sweat. Each measuring procedure can. The body is very good at keeping its temperature within a safe range even when temperatures outside the body change a lot.
Mouse body temperature measurement during anesthetization. The core body temperature of mice has been measured by either the insertion of a thermometer into the anus of the mouse or insertion of a thermocouple via the anus into the large intestine soothilletal1992wong etal1997. Abdominal temperature was measured using. The measuringdevice is then left insituuntil the temperature recorded stabilizes. At present mouse body temperature data for metabolic phenotyping is mainly obtained by one of three methods. In adult mice an insertion depth of 2 cm will yield colonic temperatures.
Gallery of Mouse Body Temperature Measurement
